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ENTH/VHS domain superfamily

SCOP classification
Root:   SCOP hierarchy in SUPERFAMILY [ 0] (11)
Class:   All alpha proteins [ 46456] (284)
Fold:   alpha-alpha superhelix [ 48370] (24)
Superfamily:   ENTH/VHS domain [ 48464] (4)
Families:   ENTH domain [ 48465]
  VHS domain [ 48468] (5)
  Phosphoinositide-binding clathrin adaptor, N-terminal domain [ 100911] (2)
  RPR domain (SMART 00582 ) [ 109975]

Superfamily statistics
Genomes (506) Uniprot 2018_03 genome PDB chains (SCOP 1.75)
Domains 9,866 27,612 14
Proteins 9,833 27,532 14

Functional annotation
General category Processes_IC
Detailed category Transport

Function annotation of SCOP domain superfamilies

Mouse Phenotype (MP)

(show details)
MP termFDR (all)SDMP levelAnnotation (direct or inherited)
Mammalian Phenotype (MP)growth/size/body region phenotype0.02845Least InformativeInherited
Mammalian Phenotype (MP)abnormal homeostasis0.9938Least InformativeInherited
Mammalian Phenotype (MP)abnormal postnatal growth/weight/body size0.1705Moderately InformativeInherited
Mammalian Phenotype (MP)abnormal glucose homeostasis0.258InformativeInherited
Mammalian Phenotype (MP)postnatal growth retardation0.000001455Highly InformativeDirect
Mammalian Phenotype (MP)decreased birth weight0.000769Highly InformativeDirect
Mammalian Phenotype (MP)hypoglycemia0.0009464Highly InformativeDirect

Document: MP annotation of SCOP domains

Worm Phenotype (WP)

(show details)
WP termFDR (all)SDWP levelAnnotation (direct or inherited)
Worm Phenotype (WP)larval lethal0Least InformativeDirect
Worm Phenotype (WP)cell physiology variant0.000136Least InformativeDirect
Worm Phenotype (WP)organism environmental stimulus response variant0.4771Least InformativeInherited
Worm Phenotype (WP)apoptosis variant0.000004847Moderately InformativeDirect
Worm Phenotype (WP)endocytic transport variant0.004594Moderately InformativeInherited
Worm Phenotype (WP)drug response variant0.008513Moderately InformativeInherited
Worm Phenotype (WP)accumulated germline cell corpses0.000000113InformativeDirect
Worm Phenotype (WP)coelomic system physiology variant0.000009008InformativeDirect
Worm Phenotype (WP)aldicarb response variant0.001314InformativeInherited
Worm Phenotype (WP)endocytic transport defect0.004238InformativeInherited
Worm Phenotype (WP)chemical resistant0.04041InformativeInherited
Worm Phenotype (WP)drug resistant0.1061InformativeInherited
Worm Phenotype (WP)coelomocyte endocytosis defective0.000007585Highly InformativeDirect
Worm Phenotype (WP)aldicarb resistant0.0004576Highly InformativeDirect

Document: WP annotation of SCOP domains

Fly Anatomy (FA)

(show details)
FA termFDR (all)SDFA levelAnnotation (direct or inherited)
Fly Anatomy (FA)cell0.4683Least InformativeInherited
Fly Anatomy (FA)multi-tissue structure0.4767Least InformativeInherited
Fly Anatomy (FA)larva0.4973Least InformativeInherited
Fly Anatomy (FA)epithelium0.1251Moderately InformativeInherited
Fly Anatomy (FA)epithelial cell0.006477InformativeInherited
Fly Anatomy (FA)imaginal disc0.01688InformativeInherited
Fly Anatomy (FA)cuboidal/columnar epithelium0.234InformativeInherited

Document: FA annotation of SCOP domains

Zebrafish Anatomy (ZA)

(show details)
ZA termFDR (all)SDZA levelAnnotation (direct or inherited)
Zebrafish Anatomy (ZA)organism subdivision0Least InformativeDirect
Zebrafish Anatomy (ZA)head0Moderately InformativeDirect

Document: ZA annotation of SCOP domains

Xenopus Anatomy (XA)

(show details) Document: XA annotation of SCOP domains

Arabidopsis Plant Ontology (AP)

(show details) Document: AP annotation of SCOP domains

InterPro annotation
Cross references IPR008942 SSF48464 Protein matches

This entry represents domains with a multi-helical, alpha-alpha 2-layered structural fold as found in: the ENTH domain of Epsin; the VHS domain of Hrs, Tom1, and ADP-ribosylation factors; the RPR domain of PCF11 protein; and the N-terminal domain of phosphoinositide-binding clathrin adaptor.

The epsin NH2-terminal homology (ENTH) domain is a membrane interacting module composed of a superhelix of alpha-helices. It is present at the NH2-terminus of proteins that often contain consensus sequences for binding to clathrin coat components and their accessory factors, and therefore function as endocytic adaptors. ENTH domain containing proteins have additional roles in signalling and actin regulation and may have yet other actions in the nucleus. The ENTH domain is structurally similar to the VHS domain.

The ENTH domain is approximately 150 amino acids long. The ENTH domain forms a compact globular structure, composed of eight alpha-helices connected by loops of varying length. Three helical hairpins that are stacked consecutively with a right-handed twist determine the general topology of the domain. This stacking gives the ENTH domain a rectangular appearance when viewed face on. The most highly conserved amino acids fall roughly into two classes: internal residues that are involved in packing and therefore are necessary for structural integrity, and solvent accessible residues that may be involved in protein-protein interactions [PubMed11911874].

VHS domains are found at the N-termini of select proteins involved in intracellular membrane trafficking. The domain consists of eight helices arranged in a superhelix. The surface of the domain has two main features: a basic patch on one side due to several conserved positively charged residues on helix 3 and a negatively charged ridge on the opposite side, formed by residues on helix 2. Comparison of the two VHS domains and the ENTH domain reveals a conserved surface, composed of helices 2 and 4, that is utilised for protein-protein interactions. In addition, VHS domain-containing proteins are also often localized to membranes. It has therefore been suggested that the conserved positively charged surface of helix 3 in VHS and ENTH domains plays a role in membrane binding [PubMed10985773].

InterPro database

PDBeMotif information about ligands, sequence and structure motifs
Cross references PDB entries
Ligand binding statistics
Nucleic-acid binding statistics
Occurrence of secondary structure elements
Occurrence of small 3D structural motifs

PDBeMotif resource

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Mouse Phenotype (MP) · Worm Phenotype (WP) · Fly Anatomy (FA) · Zebrafish Anatomy (ZA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) ]

Internal database links

Browse genome assignments for this superfamily. The SUPERFAMILY hidden Markov model library has been used to carry out SCOP domain assignments to all genomes at the superfamily level.

Alignments of sequences to 23 models in this superfamily are available by clicking on the 'Alignments' icon above. PDB sequences less than 40% identical are shown by default, but any other sequence(s) may be aligned. Select PDB sequences, genome sequences, or paste in or upload your own sequences.

Browse and view proteins in genomes which have different domain combinations including a ENTH/VHS domain domain.

Examine the distribution of domain superfamilies, or families, across the major taxonomic kingdoms or genomes within a kingdom. This gives an immediate impression of how superfamilies, or families, are restricted to certain kingdoms of life.

Explore domain occurrence network where nodes represent genomes and edges are domain architectures (shared between genomes) containing the superfamily of interest.

There are 23 hidden Markov models representing the ENTH/VHS domain superfamily. Information on how the models are built, and plots showing hydrophobicity, match emmission probabilities and insertion/deletion probabilities can be inspected.

Jump to [ Top of page · SCOP classification · InterPro annotation · PDBeMotif links · Functional annotation · Mouse Phenotype (MP) · Worm Phenotype (WP) · Fly Anatomy (FA) · Zebrafish Anatomy (ZA) · Xenopus Anatomy (XA) · Arabidopsis Plant Ontology (AP) · Internal database links ]