The spindle assembly checkpoint protein mad2 superfamily

SCOP classification

• Root: SCOP hierarchy in SUPERFAMILY [ 0] (11)
• Class: Alpha and beta proteins (a+b) [ 53931] (376)
• Fold: The spindle assembly checkpoint protein mad2 [ 56018]
• Superfamily: The spindle assembly checkpoint protein mad2 [ 56019]
• Families: The spindle assembly checkpoint protein mad2 [ 56020]

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Superfamily statistics

	Genomes (517)	Uniprot 2018_03 genome	PDB chains (SCOP 1.75)
Domains	1,677	4,457	5
Proteins	1,662	4,410	5

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Functional annotation

• General category: Processes_IC
• Detailed category: Cell cycle, Apoptosis

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Mouse Phenotype (MP)

Highlighted in gray are those with FDR_all>0.001

	MP term	FDR (all)	IC level	SDMP level	Annotation (direct or inherited)
Mammalian Phenotype (MP)	abnormal apoptosis	0	1.076	--	DIRECT
Mammalian Phenotype (MP)	abnormal cell physiology	0	0.6214	--	DIRECT
Mammalian Phenotype (MP)	abnormal oogenesis	0.00000281	1.809	--	DIRECT
Mammalian Phenotype (MP)	azoospermia	0.00000543	2.508	--	DIRECT
Mammalian Phenotype (MP)	absent gametes	0.000007099	2.207	--	DIRECT
Mammalian Phenotype (MP)	arrest of spermatogenesis	0.000009534	2.508	--	DIRECT
Mammalian Phenotype (MP)	decreased testis weight	0.00002535	2.207	--	DIRECT
Mammalian Phenotype (MP)	decreased germ cell number	0.0001889	1.605	--	DIRECT
Mammalian Phenotype (MP)	abnormal testis size	0.00029	1.809	--	DIRECT
Mammalian Phenotype (MP)	small gonad	0.0003085	1.809	--	DIRECT
Mammalian Phenotype (MP)	abnormal male germ cell morphology	0.0003175	1.663	--	DIRECT
Mammalian Phenotype (MP)	abnormal primary sex determination	0.0003417	1.73	--	DIRECT
Mammalian Phenotype (MP)	male infertility	0.0004463	2.207	--	DIRECT
Mammalian Phenotype (MP)	abnormal testis morphology	0.0005959	1.554	--	DIRECT
Mammalian Phenotype (MP)	abnormal gametogenesis	0.0008931	1.332	--	DIRECT
Mammalian Phenotype (MP)	abnormal male reproductive system morphology	0.001147	1.429	--	INHERITED FROM: abnormal testis weight || decreased testis weight || small testis || abnormal testis morphology || abnormal testis size
Mammalian Phenotype (MP)	abnormal sex gland morphology	0.001573	1.207	--	INHERITED FROM: abnormal testis size || small testis || abnormal testis morphology || abnormal male reproductive gland morphology || decreased testis weight || abnormal testis weight
Mammalian Phenotype (MP)	reproductive system phenotype	0.01695	0.9281	--	INHERITED FROM: absent germ cells || abnormal sex determination || decreased male germ cell number || abnormal male germ cell morphology || abnormal testis morphology || small gonad || abnormal spermatogenesis || decreased testis weight || small testis || decreased germ cell number || abnormal gametogenesis || abnormal oogenesis || abnormal testis size || abnormal gametes || male infertility || azoospermia || abnormal primary sex determination || arrest of spermatogenesis || abnormal sperm number || abnormal male reproductive gland morphology || abnormal testis weight || absent gametes
Mammalian Phenotype (MP)	endocrine/exocrine gland phenotype	0.02475	0.8003	--	INHERITED FROM: decreased testis weight || abnormal male reproductive gland morphology || abnormal testis size || small testis || abnormal testis weight || abnormal testis morphology
Mammalian Phenotype (MP)	abnormal male genitalia morphology	1	1.429	--	INHERITED FROM: abnormal testis weight || decreased testis weight || abnormal testis morphology || small testis || abnormal testis size
Mammalian Phenotype (MP)	cellular phenotype	0	0.5301	Least Informative	DIRECT
Mammalian Phenotype (MP)	abnormal cell death	0	1.045	Moderately Informative	DIRECT
Mammalian Phenotype (MP)	abnormal cell morphology	0	1.229	Moderately Informative	DIRECT
Mammalian Phenotype (MP)	abnormal reproductive system morphology	0.004741	1.061	Moderately Informative	INHERITED FROM: decreased germ cell number || abnormal gametogenesis || abnormal oogenesis || small testis || abnormal testis size || abnormal gametes || decreased testis weight || abnormal primary sex determination || arrest of spermatogenesis || azoospermia || abnormal male reproductive gland morphology || abnormal sperm number || absent gametes || abnormal testis weight || absent germ cells || abnormal sex determination || abnormal male germ cell morphology || decreased male germ cell number || small gonad || abnormal testis morphology || abnormal spermatogenesis
Mammalian Phenotype (MP)	abnormal reproductive system physiology	0.009397	1.186	Moderately Informative	INHERITED FROM: male infertility
Mammalian Phenotype (MP)	abnormal gland morphology	0.0199	0.8644	Moderately Informative	INHERITED FROM: abnormal testis size || small testis || decreased testis weight || abnormal male reproductive gland morphology || abnormal testis weight || abnormal testis morphology
Mammalian Phenotype (MP)	abnormal sex determination	0.0004121	1.663	Informative	DIRECT
Mammalian Phenotype (MP)	abnormal spermatogenesis	0.0004618	1.605	Informative	DIRECT
Mammalian Phenotype (MP)	abnormal male reproductive gland morphology	0.0009144	1.508	Informative	DIRECT
Mammalian Phenotype (MP)	abnormal internal male genitalia morphology	0.001058	1.429	Informative	INHERITED FROM: abnormal testis weight || abnormal testis size || abnormal testis morphology || small testis || decreased testis weight
Mammalian Phenotype (MP)	abnormal fertility/fecundity	0.00632	1.605	Informative	INHERITED FROM: male infertility
Mammalian Phenotype (MP)	abnormal germ cell morphology	0.1736	1.466	Informative	INHERITED FROM: decreased male germ cell number || abnormal gametes || absent germ cells || absent gametes || decreased germ cell number || azoospermia || abnormal male germ cell morphology
Mammalian Phenotype (MP)	absent germ cells	0.000008922	1.906	Highly Informative	DIRECT
Mammalian Phenotype (MP)	abnormal testis weight	0.00003168	2.031	Highly Informative	DIRECT
Mammalian Phenotype (MP)	abnormal sperm number	0.0001161	2.207	Highly Informative	DIRECT
Mammalian Phenotype (MP)	decreased male germ cell number	0.0001284	1.809	Highly Informative	DIRECT
Mammalian Phenotype (MP)	abnormal gametes	0.0002034	1.906	Highly Informative	DIRECT
Mammalian Phenotype (MP)	small testis	0.0002356	1.906	Highly Informative	DIRECT
Mammalian Phenotype (MP)	infertility	0.001346	2.207	Highly Informative	INHERITED FROM: male infertility
Mammalian Phenotype (MP)	abnormal male reproductive system physiology	0.001619	1.906	Highly Informative	INHERITED FROM: male infertility

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Worm Phenotype (WP)

Highlighted in gray are those with FDR_all>0.001

	WP term	FDR (all)	IC level	SDWP level	Annotation (direct or inherited)
Worm Phenotype (WP)	embryonic lethal	0	0.07108	--	DIRECT
Worm Phenotype (WP)	lethal	0	0.01121	--	DIRECT
Worm Phenotype (WP)	fertility variant	0	0.08329	--	DIRECT
Worm Phenotype (WP)	development variant	0	0.001706	--	DIRECT
Worm Phenotype (WP)	morphology variant	0	0.06117	--	DIRECT
Worm Phenotype (WP)	organism development variant	0	0.004279	--	DIRECT
Worm Phenotype (WP)	organ system physiology variant	0	0.05921	--	DIRECT
Worm Phenotype (WP)	reproductive system physiology variant	0	0.07209	--	DIRECT
Worm Phenotype (WP)	maternal sterile	0	0.171	--	DIRECT
Worm Phenotype (WP)	hermaphrodite sterile	0	0.1685	--	DIRECT
Worm Phenotype (WP)	embryonic development variant	0	0.06709	--	DIRECT
Worm Phenotype (WP)	gametogenesis variant	0	0.9517	--	DIRECT
Worm Phenotype (WP)	meiosis variant	0	1.176	--	DIRECT
Worm Phenotype (WP)	hermaphrodite fertility variant	0	0.1278	--	DIRECT
Worm Phenotype (WP)	chromosome disjunction defective	0	1.929	--	DIRECT
Worm Phenotype (WP)	hermaphrodite fertility reduced	0	0.1672	--	DIRECT
Worm Phenotype (WP)	fertility reduced	0	0.09267	--	DIRECT
Worm Phenotype (WP)	meiotic chromosome segregation variant	0	1.929	--	DIRECT
Worm Phenotype (WP)	aneuploidy	0	1.929	--	DIRECT
Worm Phenotype (WP)	physiology variant	1	0	--	INHERITED FROM: hermaphrodite fertility variant || X chromosome nondisjunction || reproductive system physiology variant || lethal || meiotic chromosome organization variant || chromosome disjunction defective || meiosis variant || germ cell development variant || cell division variant || cell homeostasis metabolism variant || development variant || chromosome segregation variant || cell physiology variant || hermaphrodite sterile || hermaphrodite fertility reduced || embryonic development variant || embryonic lethal || fertility variant || gametogenesis variant || cell cycle variant || meiotic chromosome segregation variant || organ system physiology variant || maternal sterile || organism development variant || aneuploidy || fertility reduced || cell development variant
Worm Phenotype (WP)	sterile	1	0.161	--	INHERITED FROM: hermaphrodite sterile || maternal sterile
Worm Phenotype (WP)	cell development variant	0	0.632	Least Informative	DIRECT
Worm Phenotype (WP)	cell physiology variant	0	0.5344	Least Informative	DIRECT
Worm Phenotype (WP)	cell homeostasis metabolism variant	0	0.9294	Moderately Informative	DIRECT
Worm Phenotype (WP)	cell cycle variant	0	1.164	Moderately Informative	DIRECT
Worm Phenotype (WP)	cell division variant	0	1	Moderately Informative	DIRECT
Worm Phenotype (WP)	germ cell development variant	0	0.9752	Moderately Informative	DIRECT
Worm Phenotype (WP)	chromosome segregation variant	0	1.708	Informative	DIRECT
Worm Phenotype (WP)	meiotic chromosome organization variant	0	1.503	Informative	DIRECT
Worm Phenotype (WP)	X chromosome nondisjunction	0	1.929	Highly Informative	DIRECT

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Yeast Phenotype (YP)

Highlighted in gray are those with FDR_all>0.001

	YP term	FDR (all)	IC level	SDYP level	Annotation (direct or inherited)
Yeast Phenotype (YP)	cellular processes	0	0.1328	--	DIRECT
Yeast Phenotype (YP)	stress resistance	0	0.2018	--	DIRECT
Yeast Phenotype (YP)	competitive fitness	0	0.1389	--	DIRECT
Yeast Phenotype (YP)	fitness	0	0.1328	--	DIRECT
Yeast Phenotype (YP)	metabolism and growth	0	0.5905	Least Informative	DIRECT

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Xenopus Anatomy (XA)

Highlighted in gray are those with FDR_all>0.001

	XA term	FDR (all)	IC level	SDXA level	Annotation (direct or inherited)
Xenopus ANatomical entity (XAN)	trunk region	0	0.4287	--	DIRECT
Xenopus ANatomical entity (XAN)	genital system	0	0.5909	--	DIRECT
Xenopus ANatomical entity (XAN)	male genitalia	0	0.8318	--	DIRECT
Xenopus ANatomical entity (XAN)	anatomical structure	0	0	--	DIRECT
Xenopus ANatomical entity (XAN)	anatomical group	0	0.1442	--	DIRECT
Xenopus ANatomical entity (XAN)	male organism	0	0.8318	--	DIRECT
Xenopus ANatomical entity (XAN)	cell	0	0.7974	--	DIRECT
Xenopus ANatomical entity (XAN)	organism subdivision	0	0.1871	--	DIRECT
Xenopus ANatomical entity (XAN)	compound organ	0	0.24	--	DIRECT
Xenopus ANatomical entity (XAN)	egg	0	1.17	--	DIRECT
Xenopus ANatomical entity (XAN)	gonad	0	0.8318	--	DIRECT
Xenopus ANatomical entity (XAN)	gamete	0	1.17	--	DIRECT
Xenopus ANatomical entity (XAN)	anatomical region	0	0.193	--	DIRECT
Xenopus ANatomical entity (XAN)	multicellular anatomical structure	0	0.1217	--	DIRECT
Xenopus ANatomical entity (XAN)	anatomical system	1	0.3094	--	INHERITED FROM: testis || urogenital system || egg || genital system || male genitalia || gamete
Xenopus ANatomical entity (XAN)	whole organism	1	0.09747	--	INHERITED FROM: urogenital system || genital system || egg || anatomical group || testis || cavitated compound organ || multicellular anatomical structure || gonad || compound organ || trunk || anatomical region || germ cell || male genitalia || gamete || trunk region || male organism || organism subdivision || cell
Xenopus ANatomical entity (XAN)	urogenital system	0	0.5011	Least Informative	DIRECT
Xenopus ANatomical entity (XAN)	trunk	0	0.4308	Least Informative	DIRECT
Xenopus ANatomical entity (XAN)	cavitated compound organ	0	0.3033	Least Informative	DIRECT
Xenopus ANatomical entity (XAN)	germ cell	0	1.013	Moderately Informative	DIRECT
Xenopus ANatomical entity (XAN)	testis	0	0.8318	Moderately Informative	DIRECT
Xenopus DEvelopment stage (XDE)	NF stage	0	0.02327	--	DIRECT

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Arabidopsis Plant Ontology (AP)

Highlighted in gray are those with FDR_all>0.001

	AP term	FDR (all)	IC level	SDAP level	Annotation (direct or inherited)
Plant ANatomical entity (PAN)	shoot system	0	0.05747	--	DIRECT
Plant ANatomical entity (PAN)	collective plant structure	0	0.01577	--	DIRECT
Plant ANatomical entity (PAN)	plant structure	1	0	--	INHERITED FROM: collective plant structure || shoot system
Plant ANatomical entity (PAN)	collective plant organ structure	1	0.04502	--	INHERITED FROM: shoot system
Plant structure DEvelopment stage (PDE)	whole plant development stage	0	0.1968	--	DIRECT
Plant structure DEvelopment stage (PDE)	sporophyte vegetative stage	0	0.2036	--	DIRECT
Plant structure DEvelopment stage (PDE)	floral organ differentiation stage	0	0.0937	--	DIRECT
Plant structure DEvelopment stage (PDE)	corolla development stage	0	0.1981	--	DIRECT
Plant structure DEvelopment stage (PDE)	plant embryo stage	0	0.2833	--	DIRECT
Plant structure DEvelopment stage (PDE)	collective plant organ structure development stage	0	0.03771	--	DIRECT
Plant structure DEvelopment stage (PDE)	plant organ development stage	0	0.08108	--	DIRECT
Plant structure DEvelopment stage (PDE)	reproductive shoot system development stage	0	0.05008	--	DIRECT
Plant structure DEvelopment stage (PDE)	collective phyllome structure development stage	0	0.0937	--	DIRECT
Plant structure DEvelopment stage (PDE)	floral organ formation stage	0	0.0937	--	DIRECT
Plant structure DEvelopment stage (PDE)	flower development stage	1	0.07083	--	INHERITED FROM: corolla development stage || floral organ differentiation stage || floral organ formation stage
Plant structure DEvelopment stage (PDE)	life of whole plant stage	1	0.2022	--	INHERITED FROM: sporophyte vegetative stage || E expanded cotyledon stage || D bilateral stage || plant embryo stage
Plant structure DEvelopment stage (PDE)	shoot system development stage	1	0.04053	--	INHERITED FROM: reproductive shoot system development stage || floral organ formation stage || floral organ differentiation stage || corolla development stage || plant organ development stage
Plant structure DEvelopment stage (PDE)	sporophyte development stage	1	0.2022	--	INHERITED FROM: E expanded cotyledon stage || sporophyte vegetative stage || D bilateral stage || plant embryo stage
Plant structure DEvelopment stage (PDE)	E expanded cotyledon stage	0	0.353	Least Informative	DIRECT
Plant structure DEvelopment stage (PDE)	D bilateral stage	0	0.3729	Least Informative	DIRECT

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InterPro annotation

• Cross references: IPR003511 SSF56019 Protein matches
• Abstract: The HORMA (for Hop1p, Rev7p and MAD2) domain has been suggested to recognise chromatin states that result from DNA adducts, double stranded breaks or non-attachment to the spindle and acts as an adaptor that recruits other proteins. Hop1 is a meiosis-specific protein, Rev7 is required for DNA damage induced mutagenesis, and MAD2 is a spindle checkpoint protein which prevents progression of the cell cycle upon detection of a defect in mitotic spindle integrity.

InterPro database

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